Issue 4, 2020

Enhanced avidin binding to lipid bilayers using PDP-PE lipids with PEG-biotin linkers

Abstract

Two of the most important aspects of lipid bilayers that have increased their popularity in the field of nanotechnology and biosensors are their fluid nature, which is highly beneficial in ensuring the spatial organization of attached molecules, and the relative ease in which they can be manipulated to change the surface chemistry. Here we have used two different types of functionalized lipids to study the interaction of avidin, which is a common approach to attach further ligands for study. We have tested the commonly used Biotinyl-Cap-PE lipids at different molar percentages and reveal that avidin is not evenly distributed, but forms what looks like clusters even at low percentage occupancy which hampers the level of avidin that can be associated with the surface. We have then successfully employed the novel strategy of using PDP-PE lipids which contain a reducible disulphide to which we added maleamide-PEG-biotin spacers of different lengths. There is a more even distribution of avidin on these layers and thereby increasing the amount and efficiency of avidin association. The reduced levels of avidin that was being associated with the Biotinyl-Cap-PE layers as compared to the PDP-PE lipids could be analysed with QCM-D and interferometry approaches, but it was only with SEEC microscopy that the reason for the reduced occupancy was resolved.

Graphical abstract: Enhanced avidin binding to lipid bilayers using PDP-PE lipids with PEG-biotin linkers

Supplementary files

Article information

Article type
Paper
Submitted
22 jan 2020
Accepted
07 mar 2020
First published
10 mar 2020
This article is Open Access
Creative Commons BY-NC license

Nanoscale Adv., 2020,2, 1625-1633

Enhanced avidin binding to lipid bilayers using PDP-PE lipids with PEG-biotin linkers

H. L. Birchenough, M. J. Swann, E. Zindy, A. J. Day and T. A. Jowitt, Nanoscale Adv., 2020, 2, 1625 DOI: 10.1039/D0NA00060D

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