Issue 19, 2020

The discovery of lactoferrin dual aptamers through surface plasmon resonance imaging combined with a bioinformation analysis

Abstract

An analytical method for screening aptamers for different recognition sites in lactoferrin (Lac) molecules has been developed based on Surface Plasmon Resonance imaging (SPRi), combined with the cluster classification calculation of a quasi-aptamer library strategy and molecular docking simulation analysis. Using the software simulation, a homology analysis was performed on the selected quasi-aptamer sequences, which could be divided into 8 different families. Based on the principle of biomolecular recognition, a label-free, high-throughput dual immune site screening method was established, in which the nucleic acid aptamers of recognizing ability for lactoferrin molecules were fixed onto the surface of the SPRi sensor chip and could bind to the lactoferrin molecules. Then, the aptamer candidates to be paired were introduced, and the recognition event of the second immune site was judged by observing the binding signal of SPRi. The paired SPRi signal was generated only when the site identified by the second nucleic acid molecule was different from the first immune site. Based on this principle, a pair of Lac nucleic acid aptamers (Lac-8 and Lac-25) was finally screened and confirmed using computerized simulation, and has been employed to assay Lac in milk by ELONA (Enzyme-Linked Oligonucleotide Assay).

Graphical abstract: The discovery of lactoferrin dual aptamers through surface plasmon resonance imaging combined with a bioinformation analysis

Article information

Article type
Paper
Submitted
30 jul 2020
Accepted
31 ago 2020
First published
03 set 2020

Analyst, 2020,145, 6298-6306

The discovery of lactoferrin dual aptamers through surface plasmon resonance imaging combined with a bioinformation analysis

W. Jia, Z. Wang, Z. Lu, B. Ding, Z. Li and D. Xu, Analyst, 2020, 145, 6298 DOI: 10.1039/D0AN01513J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements