Issue 7, 2019

Fluorescent peptide displacement as a general assay for screening small molecule libraries against RNA

Abstract

A prominent hurdle in developing small molecule probes against RNA is the relative scarcity of general screening methods. In this study, we demonstrate the application of a fluorescent peptide displacement assay to screen small molecule probes against four different RNA targets. The designed experimental protocol combined with statistical analysis provides a fast and convenient method to simultaneously evaluate small molecule libraries against different RNA targets and classify them based on affinity and selectivity patterns.

Graphical abstract: Fluorescent peptide displacement as a general assay for screening small molecule libraries against RNA

Supplementary files

Article information

Article type
Communication
Submitted
04 out 2018
Accepted
19 nov 2018
First published
20 nov 2018

Org. Biomol. Chem., 2019,17, 1778-1786

Fluorescent peptide displacement as a general assay for screening small molecule libraries against RNA

N. N. Patwardhan, Z. Cai, C. N. Newson and A. E. Hargrove, Org. Biomol. Chem., 2019, 17, 1778 DOI: 10.1039/C8OB02467G

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