Issue 60, 2019

Redefining the photo-stability of common fluorophores with triplet state quenchers: mechanistic insights and recent updates

Abstract

Light microscopy can offer certain advantages over electron microscopy in terms of acquiring detailed insights into the biological/intra-cellular milieu. In recent years, with the development of new fluorescence imaging technologies, it has become extremely important to assess the role of designing appropriate fluorophores in acquiring desired biological information without encountering any untoward hitches. Over the years, external fluorophores have been prevalently used in fluorescence microscopy and single-molecule fluorescence microscopy-based studies. Photostable fluorogenic probes with high extinction coefficients and quantum yields, exhibiting minimum autofluorescence and photobleaching properties, are preferred in single-molecule microscopy as they can tolerate long-term laser exposure. Therefore, the development of triplet state quenchers and/or any other suitable new strategy to ensure the photo-stability of the fluorophores during long-term live cell imaging exercises is highly anticipated. In this feature article, various strategies for stabilizing fluorophores, including the mechanisms of TSQ-induced stabilization, have been thoroughly reviewed considering contemporary literature reports and applications.

Graphical abstract: Redefining the photo-stability of common fluorophores with triplet state quenchers: mechanistic insights and recent updates

Article information

Article type
Feature Article
Submitted
04 abr 2019
Accepted
26 abr 2019
First published
26 abr 2019

Chem. Commun., 2019,55, 8695-8704

Redefining the photo-stability of common fluorophores with triplet state quenchers: mechanistic insights and recent updates

W. Gong, P. Das, S. Samanta, J. Xiong, W. Pan, Z. Gu, J. Zhang, J. Qu and Z. Yang, Chem. Commun., 2019, 55, 8695 DOI: 10.1039/C9CC02616A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements