Issue 23, 2018

Microfluidic bead encapsulation above 20 kHz with triggered drop formation

Abstract

Microsphere beads are functionalized with oligonucleotides, antibodies, and other moieties to enable specific detection of analytes. Droplet microfluidics leverages this for single-molecule or -cell analysis by pairing beads and targets in water-in-oil droplets. Pairing is achieved with devices operating in the dripping regime, limiting throughput. Here, we describe a pairing method that uses beads to trigger the breakup of a jet into monodispersed droplets. We use the method to pair 105 Human T cells with polyacrylamide beads ten times faster than methods operating in the dripping regime. Our method improves the throughput of bead-based droplet workflows, enabling analysis of large populations and the detection of rare events.

Graphical abstract: Microfluidic bead encapsulation above 20 kHz with triggered drop formation

Supplementary files

Article information

Article type
Paper
Submitted
21 mai 2018
Accepted
03 out 2018
First published
26 out 2018
This article is Open Access
Creative Commons BY-NC license

Lab Chip, 2018,18, 3598-3605

Microfluidic bead encapsulation above 20 kHz with triggered drop formation

I. C. Clark and A. R. Abate, Lab Chip, 2018, 18, 3598 DOI: 10.1039/C8LC00514A

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements