Detection of the effect of polydopamine (PDA)-coated polydimethylsiloxane (PDMS) substrates on the release of H2O2 from a single HeLa cell

Jingyu Xiao; Meng Tian; Lei Su; Yu Bao; Li Niu; Xueji Zhang

doi:10.1039/D1AN01506K

You do not have JavaScript enabled. Please enable JavaScript to access the full features of the site or access our non-JavaScript page.

Detection of the effect of polydopamine (PDA)-coated polydimethylsiloxane (PDMS) substrates on the release of H₂O₂ from a single HeLa cell†

Jingyu Xiao,^b Meng Tian,^b Lei Su,

*^a Yu Bao,*^c Li Niu

^c and Xueji Zhang

*^a

Author affiliations

* Corresponding authors

^a School of Biomedical Engineering, Health Science Center, Shenzhen University, Shenzhen 518037, P.R. China, P. R. China
E-mail: sulei@szu.edu.cn, zhangxueji@szu.edu.cn

^b School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, P. R. China

^c Center for Advanced Analytical Science, School of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou 510006, P. R. China
E-mail: baoyu@gzhu.edu.cn

Abstract

Endogenous H₂O₂ generated by a single HeLa cell that was adhered on the PDA-coated PDMS substrates under 25 mM glucose culture conditions was detected using a home-built photoelectric dual detection platform. With PMA as the stimulus, the cell released a small amount of H₂O₂ and its mitochondrial membrane potential (MMP) decrease was smaller, compared with that on the PDMS substrates.

This article is part of the themed collection: Analyst HOT Articles 2021

Download options Please wait...

Supplementary files

Supplementary information PDF (719K)

Article information

DOI: https://doi.org/10.1039/D1AN01506K
Article type: Communication
Submitted: 19 ago 2021
Accepted: 07 set 2021
First published: 08 set 2021

Download Citation

Analyst, 2021,146, 6445-6449

Permissions

Request permissions

Detection of the effect of polydopamine (PDA)-coated polydimethylsiloxane (PDMS) substrates on the release of H₂O₂ from a single HeLa cell

J. Xiao, M. Tian, L. Su, Y. Bao, L. Niu and X. Zhang, Analyst, 2021, 146, 6445 DOI: 10.1039/D1AN01506K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Social activity

Fetching data from CrossRef.
This may take some time to load.

Analyst