Issue 6, 2020

Induction of crystal nucleation by orientation-controlled binding of His6-tagged proteins to functionalized gold nanoparticles

Abstract

Protein crystallization is an indispensable process in structural determination by crystallography and consists of nucleation and crystal growth. Nucleation, wherein protein monomers in solution cluster in an ordered fashion to form a stable nucleus, is a critical yet difficult step in crystallization. Herein, we utilized sphere-, star-, and rod-shaped gold nanoparticles (GNPs) functionalized with nitrilotriacetic acid (NTA) or citrate as nucleation cores and showed that the GNPs could facilitate nucleation by interacting with His6-tagged proteins. Evaluation of the crystallization of two proteins indicated that GNPs significantly increased the number of crystallization conditions compared to that of the control. Furthermore, NTA-modified GNPs had better effect on crystallization than did citrate-capped GNPs, which could be attributed to orientation-controlled protein clustering around the GNPs by specific conjugation of NTA-modified GNP–Ni2+–His6-tagged proteins. Our findings indicate that functionalized GNPs can greatly increase the success rate of protein crystallization and accelerate the structural determination process by X-ray crystallography.

Graphical abstract: Induction of crystal nucleation by orientation-controlled binding of His6-tagged proteins to functionalized gold nanoparticles

Supplementary files

Article information

Article type
Paper
Submitted
12 nov 2019
Accepted
07 jan 2020
First published
08 jan 2020

CrystEngComm, 2020,22, 1032-1040

Induction of crystal nucleation by orientation-controlled binding of His6-tagged proteins to functionalized gold nanoparticles

J. Park, T. H. Kang, I. Choi and J. Choe, CrystEngComm, 2020, 22, 1032 DOI: 10.1039/C9CE01786K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements