Here we study mechanistic principles of the membrane-bound alternative oxidase (AOX) from Trypanosoma brucei, elucidating how AOX activates dioxygen to drive quinol oxidation by proton-coupled electron transfer reactions and electric field effects.
Complex I is an essential membrane protein in respiration and contributes to ATP synthesis. Zeta potential measurements are shown to be sensitive to the enzyme’s activity and capability to pump protons in varying lipid environments.
Vanillin-sensitive promoters were screened and employed to improve the heterologous expression of laccase in Pichia pastoris. The recombinant yeast could well remove phenolic compounds to improve the fermentability of wheat straw hydrolysate.
A 5 mg L−1 PAA/H2O2 (mixture) pre-oxidation decreased the DBP formation potential by up to 40%.
A schematic showing the steam, microwave and pulsed light assemblies used for the decontamination of red chillies and stored polypropylene at 28 and 4 °C.