Issue 39, 2021

Synthesis and DNA interaction studies of Ru(ii) cell penetrating peptide (CPP) bioconjugates

Abstract

The synthesis of the first bioconjugates of a set of ruthenium(II) dipyridophenazine complexes with two different cell penetrating peptides (CPPs) is described. The CPPs, an arginine rich TAT-9 (RKKRRQRRR) sequence and the Xentry peptide (LCLRPVG), were synthesized using standard SPPS protocols, and the bioconjugates were obtained by the microwave-assisted coupling of the HOBt/TBTU preactivated metal complexes with the respective peptides on Wang resin. The racemic metal complexes were obtained by modified literature procedures. The bioconjugates were cleaved from the resin, purified by semi-preparative HPLC and characterized by analytical HPLC, high resolution mass spectrometry (HR-MS), and NMR spectroscopy. Despite the bioconjugation of the peptides to the dppz ligand, DNA intercalation was observed by CD spectroscopy, viscometry and the characteristic switch-on fluorescence of this class of compounds. Furthermore, the cellular uptake of the Xentry bioconjugates was confirmed by live cell imaging. Like the parent metal complexes, the bioconjugates show low in vitro cytotoxicity (IC50 > 80 μM), which is similar to the respective metal complexes alone.

Graphical abstract: Synthesis and DNA interaction studies of Ru(ii) cell penetrating peptide (CPP) bioconjugates

Supplementary files

Article information

Article type
Paper
Submitted
31 mai 2021
Accepted
12 set 2021
First published
22 set 2021

Dalton Trans., 2021,50, 13768-13777

Synthesis and DNA interaction studies of Ru(II) cell penetrating peptide (CPP) bioconjugates

D. Obitz, R. G. Miller and N. Metzler-Nolte, Dalton Trans., 2021, 50, 13768 DOI: 10.1039/D1DT01776D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements