Issue 12, 2023

Single-chain multicolor-reporter templates for subcellular localization of molecular events in mammalian cells

Abstract

Single-chain multicolor-reporter imaging templates were developed for the subcellular localization of molecular events in mammalian cells. The templates were constructed by tandem linkage of fluorescent protein variants – fused with luciferases and the subcellular localization signal peptides. The templates simultaneously reported steroid hormonal activities at different optical spectra in the subcellular compartments. The templates contribute to the expansion of a toolbox of optical probes for subcellular localization of molecular events in intact cells.

Graphical abstract: Single-chain multicolor-reporter templates for subcellular localization of molecular events in mammalian cells

Supplementary files

Article information

Article type
Communication
Submitted
12 jul 2023
Accepted
25 set 2023
First published
03 out 2023
This article is Open Access
Creative Commons BY-NC license

RSC Chem. Biol., 2023,4, 1043-1049

Single-chain multicolor-reporter templates for subcellular localization of molecular events in mammalian cells

S. Kim, R. Paulmurugan, N. Kitada and S. A. Maki, RSC Chem. Biol., 2023, 4, 1043 DOI: 10.1039/D3CB00077J

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