Issue 2, 2021

Crosslinker-modified nucleic acid probes for improved target identification and biomarker detection

Abstract

Understanding the intricate interaction pattern of nucleic acids with other molecules is essential to gain further insight in biological processes and disease mechanisms. To this end, a multitude of hybridization-based assays have been designed that rely on the non-covalent recognition between complementary nucleic acid sequences. However, the ephemeral nature of these interactions complicates straightforward analysis as low efficiency and specificity are rule rather than exception. By covalently locking nucleic acid interactions by means of a crosslinking agent, the overall efficiency, specificity and selectivity of hybridization-based assays could be increased. In this mini-review we highlight methodologies that exploit the use of crosslinker-modified nucleic acid probes for interstrand nucleic acid crosslinking with the objective to study, detect and identify important targets as well as nucleic acid sequences that can be considered relevant biomarkers. We emphasize on the usefulness and advantages of crosslinking agents and elaborate on the chemistry behind the crosslinking reactions they induce.

Graphical abstract: Crosslinker-modified nucleic acid probes for improved target identification and biomarker detection

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Article information

Article type
Review Article
Submitted
22 dez 2020
Accepted
11 fev 2021
First published
22 fev 2021
This article is Open Access
Creative Commons BY-NC license

RSC Chem. Biol., 2021,2, 410-422

Crosslinker-modified nucleic acid probes for improved target identification and biomarker detection

J. Elskens and A. Madder, RSC Chem. Biol., 2021, 2, 410 DOI: 10.1039/D0CB00236D

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