Issue 22, 2018

A novel EI-GC/MS method for the accurate quantification of anti-aging compound oleoylethanolamine in C. elegans

Abstract

Endocannabinoids and related N-acyl ethanolamine-derived lipids affect a diverse array of physiological processes and pathological conditions. In the roundworm C. elegans, several endocannabinoid-like molecules have been implicated in regulating axon regeneration, energy balance and food intake as well as aging. One such molecule oleoylethanolamine (OEA) has been shown to promote life extension through nuclear receptor signal transduction, and its accurate quantitation therefore is of high interest. Using a combination of electron impact ionization (EI) and collision induced dissociation coupled to gas chromatography (GC), we found unique fragmentation ions of OEA and designed a specific MRM method for its accurate quantification. Our method should provide a reproducible and robust way to measure OEA dynamics under different genetic, pharmacologic and environmental perturbations.

Graphical abstract: A novel EI-GC/MS method for the accurate quantification of anti-aging compound oleoylethanolamine in C. elegans

Supplementary files

Article information

Article type
Paper
Submitted
22 fev 2018
Accepted
24 abr 2018
First published
30 abr 2018
This article is Open Access
Creative Commons BY license

Anal. Methods, 2018,10, 2551-2559

A novel EI-GC/MS method for the accurate quantification of anti-aging compound oleoylethanolamine in C. elegans

A. Annibal, Ö. Karalay, C. Latza and A. Antebi, Anal. Methods, 2018, 10, 2551 DOI: 10.1039/C8AY00396C

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements