Issue 37, 2017

A novel colorimetric assay for α-methylacyl-CoA racemase 1A (AMACR; P504S) utilizing the elimination of 2,4-dinitrophenolate

Abstract

α-Methylacyl-CoA racemase (AMACR; P504S) regulates branched-chain fatty acid degradation, activates Ibuprofen and is a recognised cancer drug target. A novel, facile colorimetric assay was developed based on elimination of 2,4-dinitrophenolate. The assay was used to test 5 known inhibitors, determining IC50 and Ki values, reversibility and characterizing irreversible inhibition.

Graphical abstract: A novel colorimetric assay for α-methylacyl-CoA racemase 1A (AMACR; P504S) utilizing the elimination of 2,4-dinitrophenolate

Supplementary files

Article information

Article type
Communication
Submitted
18 jan 2017
Accepted
17 mar 2017
First published
17 mar 2017
This article is Open Access
Creative Commons BY license

Chem. Commun., 2017,53, 5087-5090

A novel colorimetric assay for α-methylacyl-CoA racemase 1A (AMACR; P504S) utilizing the elimination of 2,4-dinitrophenolate

M. Yevglevskis, G. L. Lee, A. Nathubhai, Y. D. Petrova, T. D. James, M. D. Threadgill, T. J. Woodman and M. D. Lloyd, Chem. Commun., 2017, 53, 5087 DOI: 10.1039/C7CC00476A

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