Diversify your bonds! The reactivity of preactivated cycloalkynes in the retro-Cope elimination reaction was quantum chemically investigated. Based on our finding, we rationally designed a suite of next-generation reagents.
In this review, we present six methodologies for the synthesis of cyclooctyne derivatives, which are important reactants in bioorthogonal chemistry. Key aspects like the broad applicability and feasibility of these synthetic pathways are discussed.
Heterocyclic scaffold-fusion of dimethoxy-DIBO yields photoactivatable pyrrolidine derivatives with efficient SPAAC reactivity, whereas triazole fusions produce nonreactive fluorophores.
We report the synthesis of heterobiarylcyclooctynes bearing an endocyclic heteroatom, oxa-azabenzobenzocyclooctynes (O-ABCs).
This article provides a comprehensive overview of recent advances in the development of bioorthogonally activated probes for labelling and precise in situ fluorescence imaging both in vitro and in vivo.