Recent advances in biological nanopores for nanopore sequencing, sensing and comparison of functional variations in MspA mutants

Huma Bhatti, Rohil Jawed, Irshad Ali, Khurshid Iqbal, Yan Han, Zuhong Lu and Quanjun Liu

RSC Adv., 2021, 11, 28996
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Controlled movement of ssDNA conjugated peptide through Mycobacterium smegmatis porin A (MspA) nanopore by a helicase motor for peptide sequencing application

Zhijie Chen, Zhenqin Wang, Yang Xu, Xiaochun Zhang, Boxue Tian and Jingwei Bai

Chem. Sci., 2021, 12, 15750
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Versatility of threose nucleic acids: synthesis, properties, and applications in chemical biology and biomedical advancements

Dick Yan Tam, Pan Li, Ling Sum Liu, Fei Wang, Hoi Man Leung and Pik Kwan Lo

Chem. Commun., 2024, 60, 11864
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Compatibility of 5-ethynyl-2′F-ANA UTP with in vitro selection for the generation of base-modified, nuclease resistant aptamers

Fabienne Levi-Acobas, Adam Katolik, Pascal Röthlisberger, Thomas Cokelaer, Ivo Sarac, Masad J. Damha, Christian J. Leumann and Marcel Hollenstein

Org. Biomol. Chem., 2019, 17, 8083
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Upconversion nanoparticle and gold nanocage satellite assemblies for sensitive ctDNA detection in serum

Jiawei Wang, Guanping Hua, Lihuang Li, Danyang Li, Fanfan Wang, Jiazhen Wu, Ziyue Ye, Xi Zhou, Shefang Ye, Jun Yang, Xinjie Zhang and Lei Ren

Analyst, 2020, 145, 5553
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Rapid and multiplex preparation of engineered Mycobacterium smegmatis porin A (MspA) nanopores for single molecule sensing and sequencing

Shuanghong Yan, Liying Wang, Xiaoyu Du, Shanyu Zhang, Sha Wang, Jiao Cao, Jinyue Zhang, Wendong Jia, Yuqin Wang, Panke Zhang, Hong-Yuan Chen and Shuo Huang

Chem. Sci., 2021, 12, 9339
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Single molecule observation of hard–soft-acid–base (HSAB) interaction in engineeredMycobacterium smegmatisporin A (MspA) nanopores

Sha Wang, Jiao Cao, Wendong Jia, Weiming Guo, Shuanghong Yan, Yuqin Wang, Panke Zhang, Hong-Yuan Chen and Shuo Huang

Chem. Sci., 2020, 11, 879
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Direct microRNA Sequencing Using Nanopore-Induced Phase-Shift Sequencing

Jinyue Zhang, Shuanghong Yan, Le Chang, Weiming Guo, Yuqin Wang, Yu Wang, Panke Zhang, Hong-Yuan Chen and Shuo Huang

iScience, 2020, 23, 100916
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Xeno Nucleic Acids as Functional Materials: From Biophysical Properties to Application

Tianyuan Bian, Yufeng Pei, Shitao Gao, Songtao Zhou, Xinyu Sun, Mingdong Dong and Jie Song

Adv Healthcare Materials, 2024, 13
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Design Strategies toward Precise Protein Sequencing with Biological Nanopores

Ying-Yuan Cui, Lei Yang, Jian-Nan Zhao, Wen-Chuan Lai and Zhi-Yuan Gu

Anal. Chem., 2026, 98, 1
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SERS Detection of Nucleobases in Single Silver Plasmonic Nanopores

Jiao Cao, Hai-Ling Liu, Jin-Mei Yang, Zhong-Qiu Li, Dong-Rui Yang, Li-Na Ji, Kang Wang and Xing-Hua Xia

ACS Sens., 2020, 5, 2198
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Biological Nanopores: Engineering on Demand

Ana Crnković, Marija Srnko and Gregor Anderluh

Life, 2021, 11, 27
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A Nanopore‐Based Saccharide Sensor

Shanyu Zhang, Zhenyuan Cao, Pingping Fan, Yuqin Wang, Wendong Jia, Liying Wang, Kefan Wang, Yao Liu, Xiaoyu Du, Chengzhen Hu, Panke Zhang, Hong‐Yuan Chen and Shuo Huang

Angew Chem Int Ed, 2022, 61
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Nanopore Deciphering Single Digital Polymers Towards High‐Density Data Storage

Zheng‐Li Hu, Yu‐Hang Liu, Kai‐Li Xin, Ru‐Jia Yu, Li‐Min Zhang and Yi‐Lun Ying

Chemistry A European J, 2023, 29
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Nanopore Sequencing Accurately Identifies the Mutagenic DNA Lesion O6‐Carboxymethyl Guanine and Reveals Its Behavior in Replication

Yu Wang, Kiran M. Patil, Shuanghong Yan, Panke Zhang, Weiming Guo, Yuqin Wang, Hong‐Yuan Chen, Dennis Gillingham and Shuo Huang

Angew Chem Int Ed, 2019, 58, 8432
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Ultrasensitive Nanopore Sensing of Mucin 1 and Circulating Tumor Cells in Whole Blood of Breast Cancer Patients by Analyte-Triggered Triplex-DNA Release

Ke Sun, Piaopiao Chen, Shixin Yan, Weidan Yuan, Yu Wang, Xinqiong Li, Linqin Dou, Changjian Zhao, Jianfu Zhang, Qiang Wang, Zhoukai Fu, Long Wei, Zhaodan Xin, Zhuoyun Tang, Yichen Yan, Yiman Peng, Binwu Ying, Jie Chen and Jia Geng

ACS Appl. Mater. Interfaces, 2021, 13, 21030
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Nanopore Detection Assisted DNA Information Processing

Zichen Song, Yuan Liang and Jing Yang

Nanomaterials, 2022, 12, 3135
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A solid-phase enzymatic synthesis platform for the facile production of 2′-fluoroarabinonucleic acid (FANA) and chimeric XNA oligonucleotides using an evolved XNA polymerase

Binliang Zhang, Yuhui Du, Jingxing Zhang, Xingyun Ma, Yanjia Qin, Rui Tao, Minglan Luo, Jing Wu, Leping Sun, Gan Zhu, Hantao Luo, Junlin Wen, Chenghe Xiong, Hui Mei and Tingjian Chen

Nucleic Acids Research, 2025, 53
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Discrimination between Different DNA Lesions by Monitoring Single-Molecule Polymerase Stalling Kinetics during Nanopore Sequencing

Jinyue Zhang, Yu Wang, Yuqin Wang, Panke Zhang, Hong-Yuan Chen and Shuo Huang

Nano Lett., 2022, 22, 5561
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Chemical Modifications for a Next Generation of Nucleic Acid Aptamers

Kwing Yeung Chan, Andrew Brian Kinghorn, Marcel Hollenstein and Julian Alexander Tanner

ChemBioChem, 2022, 23
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Accurate Mapping of 5-Glyceryl-methylcytosine Using Nanopore Sequencing

Linting Wang, Baodan He, Zixin Wang, Liting Kang, Jianhuang Xue and Shuanghong Yan

Anal. Chem., 2025, 97, 9992
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Nanopore Sequencing Accurately Identifies the Mutagenic DNA Lesion O6‐Carboxymethyl Guanine and Reveals Its Behavior in Replication

Yu Wang, Kiran M. Patil, Shuanghong Yan, Panke Zhang, Weiming Guo, Yuqin Wang, Hong‐Yuan Chen, Dennis Gillingham and Shuo Huang

Angewandte Chemie, 2019, 131, 8520
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Electrode-free nanopore sensing by DiffusiOptoPhysiology

Yuqin Wang, Yu Wang, Xiaoyu Du, Shuanghong Yan, Panke Zhang, Hong-Yuan Chen and Shuo Huang

Sci. Adv., 2019, 5
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Retarded Translocation of Nucleic Acids through α-Hemolysin Nanopore in the Presence of a Calcium Flux

Sha Wang, Yuqin Wang, Shuanghong Yan, Xiaoyu Du, Panke Zhang, Hong-Yuan Chen and Shuo Huang

ACS Appl. Mater. Interfaces, 2020, 12, 26926
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A general temperature-guided language model to design proteins of enhanced stability and activity

Fan Jiang, Mingchen Li, Jiajun Dong, Yuanxi Yu, Xinyu Sun, Banghao Wu, Jin Huang, Liqi Kang, Yufeng Pei, Liang Zhang, Shaojie Wang, Wenxue Xu, Jingyao Xin, Wanli Ouyang, Guisheng Fan, Lirong Zheng, Yang Tan, Zhiqiang Hu, Yi Xiong, Yan Feng, Guangyu Yang, Qian Liu, Jie Song, Jia Liu, Liang Hong and Pan Tan

Sci. Adv., 2024, 10
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Sanger Sequencing of Xeno‐Nucleic Acid

Yueyao Wang, Ze Zhang, Lekang Chen, Jing Zhu, Shunfu Qin, Dongying Wei, Jibao Zhang, Feng Han, Fulong Wang, Qi Lin, Rui Fan, Xintong Li and Hanyang Yu

Angewandte Chemie, 2026, 138
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Single Molecule Ratcheting Motion of Peptides in a Mycobacterium smegmatis Porin A (MspA) Nanopore

Shuanghong Yan, Jinyue Zhang, Yu Wang, Weiming Guo, Shanyu Zhang, Yao Liu, Jiao Cao, Yuqin Wang, Liying Wang, Fubo Ma, Panke Zhang, Hong-Yuan Chen and Shuo Huang

Nano Lett., 2021, 21, 6703
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Aptamer-Functionalized Interface Nanopores Enable Amino Acid-Specific Peptide Detection

Tilman Schlotter, Tom Kloter, Julian Hengsteler, Kyungae Yang, Lijian Zhan, Sujeni Ragavan, Haiying Hu, Xinyu Zhang, Jens Duru, János Vörös, Tomaso Zambelli and Nako Nakatsuka

ACS Nano, 2024, 18, 6286
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Engineering Biological Nanopore Approaches toward Protein Sequencing

Xiaojun Wei, Tadas Penkauskas, Joseph E. Reiner, Celeste Kennard, Mark J. Uline, Qian Wang, Sheng Li, Aleksei Aksimentiev, Joseph W. F. Robertson and Chang Liu

ACS Nano, 2023, 17, 16369
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Sanger Sequencing of Xeno‐Nucleic Acid

Yueyao Wang, Ze Zhang, Lekang Chen, Jing Zhu, Shunfu Qin, Dongying Wei, Jibao Zhang, Feng Han, Fulong Wang, Qi Lin, Rui Fan, Xintong Li and Hanyang Yu

Angew Chem Int Ed, 2026, 65
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2′-Fluoro-arabinonucleic Acid (FANA): A Versatile Tool for Probing Biomolecular Interactions

Roberto El-Khoury and Masad J. Damha

Acc. Chem. Res., 2021, 54, 2287
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Advances and challenges in non-canonical nucleic acids data storage

Yan Wang, Yufeng Pei, Linlin Tang, Xinyu Sun, Songtao Zhou and Jie Song

Nat Commun, 2026, 17
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Real-time label-free detection of dynamic aptamer–small molecule interactions using a nanopore nucleic acid conformational sensor

Rugare G. Chingarande, Kai Tian, Yu Kuang, Aby Sarangee, Chengrui Hou, Emily Ma, Jarett Ren, Sam Hawkins, Joshua Kim, Ray Adelstein, Sally Chen, Kevin D. Gillis and Li-Qun Gu

Proc. Natl. Acad. Sci. U.S.A., 2023, 120
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Improving aptamer performance with nucleic acid mimics: de novo and post-SELEX approaches

Ricardo Oliveira, Eva Pinho, Ana Luísa Sousa, Jeffrey J. DeStefano, Nuno Filipe Azevedo and Carina Almeida

Trends in Biotechnology, 2022, 40, 549
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Giant single molecule chemistry events observed from a tetrachloroaurate(III) embedded Mycobacterium smegmatis porin A nanopore

Jiao Cao, Wendong Jia, Jinyue Zhang, Xiumei Xu, Shuanghong Yan, Yuqin Wang, Panke Zhang, Hong-Yuan Chen and Shuo Huang

Nat Commun, 2019, 10
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Modified nucleic acids: replication, evolution, and next-generation therapeutics

Karen Duffy, Sebastian Arangundy-Franklin and Philipp Holliger

BMC Biol, 2020, 18
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Nanopore Sequencing Accurately Identifies the Cisplatin Adduct on DNA

Fubo Ma, Shuanghong Yan, Jinyue Zhang, Yu Wang, Liying Wang, Yuqin Wang, Shanyu Zhang, Xiaoyu Du, Panke Zhang, Hong-Yuan Chen and Shuo Huang

ACS Sens., 2021, 6, 3082
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Unambiguous discrimination of all 20 proteinogenic amino acids and their modifications by nanopore

Kefan Wang, Shanyu Zhang, Xiao Zhou, Xian Yang, Xinyue Li, Yuqin Wang, Pingping Fan, Yunqi Xiao, Wen Sun, Panke Zhang, Wenfei Li and Shuo Huang

Nat Methods, 2024, 21, 92
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A Nanopore‐Based Saccharide Sensor

Shanyu Zhang, Zhenyuan Cao, Pingping Fan, Yuqin Wang, Wendong Jia, Liying Wang, Kefan Wang, Yao Liu, Xiaoyu Du, Chengzhen Hu, Panke Zhang, Hong‐Yuan Chen and Shuo Huang

Angewandte Chemie, 2022, 134
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New chemistries and enzymes for synthetic genetics

Niklas Freund, Maximilian Josef Ludwig Johannes Fürst and Philipp Holliger

Current Opinion in Biotechnology, 2022, 74, 129
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