SecScan: a general approach for mapping disulfide bonds in synthetic and recombinant peptides and proteins†
Stepan S.
Denisov, 
a
Johannes H.
Ippel,
a
Ben J.
Mans,
bc
Ingrid
Dijkgraaf
‡a
and
Tilman M.
Hackeng
‡*a
a
Johannes H.
Ippel,
a
Ben J.
Mans,
bc
Ingrid
Dijkgraaf
‡a
and
Tilman M.
Hackeng
‡*a
Abstract
Selenocysteine scanning (SecScan) is a novel technique to map disulfide networks in proteins independent of structure-based distance information and mass spectrometry. SecScan applies systematic substitution of single Cys by Sec in combination with NMR spectroscopy for reliable and unambiguous determination of disulfide bond networks.
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