A fluorene-conjugated diazirine is described, for which two-photon activation is demonstrated for the first time.
Linear diazirines can produce relatively long-lived diazoalkanes which are also capable of labeling proteins whereas cyclobutanediazirines are proposed to give minimal diazo product and labeling occurs predominantly via a carbene mechanism.
Crosslinking in gas-phase ions, augmented by tandem mass spectrometry and Born–Oppenheimer molecular dynamics calculations, provides analysis of structure and intermolecular interactions in peptide–peptide, peptide–nucleotide, and peptide–ligand complexes.
Photochemically induced generation of aryl fluorocarbenes from aryl fluorodiazirines and their subsequent [2+1] cycloaddition with alkenes was developed in continuous flow.
A novel, divergent radical cyclization of homoallylic diazirines for the synthesis of pyrrolines and fused diaziridines is described.