Lite Version|Standard version

To gain access to this content please
Log in via your home Institution.
Log in with your member or subscriber username and password.
Download

This paper reports label free sub-femtomolar detection of cardiac biomarker troponin T using ZnSnO3 perovskite nanomaterial based electrochemical biosensors. Herein, we have demonstrated two separate synthesis schemes for the perovskite material, one being a hydrothermal synthesis method, and the other being electrochemical deposition. In this work, for the electrochemical detection of troponin T with the hydrothermally synthesized ZnSnO3, we have used a glassy carbon electrode (GCE), on to which the nanomaterials are dropcasted so as to create a heterogeneous working electrode. In a separate scheme, we have used indium tin oxide coated polyethylene terephthalate (ITO/PET) substrates as the working electrodes, on to which a thin film of ZnSnO3 nanomaterial has been deposited electrochemically. Subsequently, the capture antibodies corresponding to the targeted cardiac troponin T are immobilized on to the surface functionalized working electrodes using NHS/EDC chemistry. The electrochemical detection of the target analyte has been performed in the concentration range of 1 fg mL−1 to 1 μg mL−1, using electrochemical impedance spectroscopy. For the GCE and ITO/PET based bioelectrodes, the approximated limits of detection are found to be 0.187 fg mL−1 and 0.571 fg mL−1 respectively, whereas the respective values of sensitivity of the same are 35.25 kΩ (μg mL−1)−1 cm−2 and 8.813 kΩ (μg mL−1)−1 cm−2.

Graphical abstract: Label free electrochemical detection of cardiac biomarker troponin T using ZnSnO3 perovskite nanomaterials

Page: ^ Top