From the journal:

Chemical Communications

A lanthanide tag for a complementary set of pseudocontact shifts

Lydia Topping,   Adarshi P. Welegedara,   Martyna Judd,   Elwy Hassan Abdelkader,   Nicholas Cox,   Gottfried Otting  and  Stephen Jonathan Butler  

Abstract

Pseudocontact shifts (PCS) generated by paramagnetic lanthanide ions deliver powerful restraints for protein structure analysis by NMR spectroscopy. We present a new lanthanide tag that generates different PCSs than that of a related tag, which differs in structure by a single oxygen atom. It is highly reactive towards cysteine and performs well in turn-on luminescence and in EPR spectroscopy.

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DOI
https://doi.org/10.1039/D4CC03007A
Article type
Communication
Submitted
20 Wax 2024
Accepted
16 Ado 2024
First published
16 Ado 2024
This article is Open Access
Creative Commons BY license

Chem. Commun., 2024, Accepted Manuscript

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A lanthanide tag for a complementary set of pseudocontact shifts

L. Topping, A. P. Welegedara, M. Judd, E. H. Abdelkader, N. Cox, G. Otting and S. J. Butler, Chem. Commun., 2024, Accepted Manuscript , DOI: 10.1039/D4CC03007A

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