Issue 35, 2019

Impact of magnetic nanoparticle surface coating on their long-term intracellular biodegradation in stem cells

Abstract

Magnetic nanoparticles (MNPs) internalized within stem cells have paved the way for remote magnetic cell manipulation and imaging in regenerative medicine. A full understanding of their interactions with stem cells and of their fate in the intracellular environment is then required, in particular with respect to their surface coatings. Here, we investigated the biological interactions of MNPs composed of an identical magnetic core but coated with different molecules: phosphonoacetic acid, polyethylene glycol phosphonic carboxylic acid, caffeic acid, citric acid, and polyacrylic acid. These coatings vary in the nature of the chelating function, the number of binding sites, and the presence or absence of a polymer. The nanoparticle magnetism was systematically used as an indicator of their internalization within human stem cells and of their structural long-term biodegradation in a 3D stem cell spheroid model. Overall, we evidence that the coating impacts the aggregation status of the nanoparticles and subsequently their uptake within stem cells, but it has little effect on their intracellular degradation. Only a high number of chelating functions (polyacrylic acid) had a significant protective effect. Interestingly, when the nanoparticles aggregated prior to cellular internalization, less degradation was also observed. Finally, for all coatings, a robust dose-dependent intracellular degradation rate was demonstrated, with higher doses of internalized nanoparticles leading to a lower degradation extent.

Graphical abstract: Impact of magnetic nanoparticle surface coating on their long-term intracellular biodegradation in stem cells

Supplementary files

Article information

Article type
Paper
Submitted
03 jul 2019
Accepted
04 aug 2019
First published
05 aug 2019

Nanoscale, 2019,11, 16488-16498

Impact of magnetic nanoparticle surface coating on their long-term intracellular biodegradation in stem cells

A. Plan Sangnier, A. B. Van de Walle, A. Curcio, R. Le Borgne, L. Motte, Y. Lalatonne and C. Wilhelm, Nanoscale, 2019, 11, 16488 DOI: 10.1039/C9NR05624F

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements