Issue 47, 2018

An injectable double-network hydrogel for the co-culture of vascular endothelial cells and bone marrow mesenchymal stem cells for simultaneously enhancing vascularization and osteogenesis

Abstract

The achievement of rapid vascularization in large implanted constructs is a major challenge in the field of bone tissue engineering. Although co-culture of bone-forming cells and vascular endothelial cells (VECs) has been expected to be a way of promoting vascularization during bone formation with a scaffold, there is a lack of detailed knowledge about the direct interactions between two types of stem cells in a three-dimensional (3D) extracellular matrix (ECM). Herein, we report on the use of an injectable cytocompatible double-network (DN) hydrogel to encapsulate, co-culture and subsequently stimulate the angiogenic/osteogenic differentiation of VECs and the human bone marrow mesenchymal stem cells (BM-MSCs), which demonstrates that the direct co-cultured system enables simultaneous enhancement of vascularization and osteogenesis by providing 3D cell–cell communication. Besides, the improved mechanical properties and the injectability of the DN hydrogel allow the delivery, long-time implantation, proliferation and differentiation of stem cells in vivo. Therefore, this study could provide a niche-like native ECM for stem cell survival and the regulation of the differentiation of multiple cell lines which will benefit bone repair.

Graphical abstract: An injectable double-network hydrogel for the co-culture of vascular endothelial cells and bone marrow mesenchymal stem cells for simultaneously enhancing vascularization and osteogenesis

Supplementary files

Article information

Article type
Paper
Submitted
25 aug 2018
Accepted
04 okt 2018
First published
05 okt 2018

J. Mater. Chem. B, 2018,6, 7811-7821

An injectable double-network hydrogel for the co-culture of vascular endothelial cells and bone marrow mesenchymal stem cells for simultaneously enhancing vascularization and osteogenesis

C. Yang, B. Han, C. Cao, D. Yang, X. Qu and X. Wang, J. Mater. Chem. B, 2018, 6, 7811 DOI: 10.1039/C8TB02244E

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