Issue 1, 2011

Making gold nanoparticles fluorescent for simultaneous absorption and fluorescence detection on the single particle level

Abstract

We demonstrate a simple way of making individual 20 nm gold nanoparticles fluorescent (with a fluorescence quantum yield of about 10−6) in glycerol. Gold NPs prepared in such a way have bright fluorescence for a long time under moderate excitation, and their fluorescence remains when the solvent is exchanged to water. We propose to use these nanoparticles as a calibration standard for simultaneousdetection of fluorescence and absorption (by means of photothermal detection), and experimentally demonstrate the theoretically predicted shift in axial positions of these signals. Simultaneous absorption and fluorescence detection of such stable labels makes them attractive for multidimensional tracking and screening applications.

Graphical abstract: Making gold nanoparticles fluorescent for simultaneous absorption and fluorescence detection on the single particle level

Supplementary files

Article information

Article type
Paper
Submitted
01 aug 2010
Accepted
01 okt 2010
First published
01 nov 2010

Phys. Chem. Chem. Phys., 2011,13, 149-153

Making gold nanoparticles fluorescent for simultaneous absorption and fluorescence detection on the single particle level

A. Gaiduk, P. V. Ruijgrok, M. Yorulmaz and M. Orrit, Phys. Chem. Chem. Phys., 2011, 13, 149 DOI: 10.1039/C0CP01389G

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