Issue 15, 2024

Laser capture microdissection and native mass spectrometry for spatially-resolved analysis of intact protein assemblies in tissue

Abstract

Previously, we have shown that native ambient mass spectrometry imaging allows the spatial mapping of folded proteins and their complexes in thin tissue sections. Subsequent top-down native ambient mass spectrometry of adjacent tissue section enables protein identification. The challenges associated with protein identification by this approach are (i) the low abundance of proteins in tissue and associated long data acquisition timescales and (ii) irregular spatial distributions which hamper targeted sampling of the relevant tissue location. Here, we demonstrate that these challenges may be overcome through integration of laser capture microdissection in the workflow. We show identification of intact protein assemblies in rat liver tissue and apply the approach to identification of proteins in the granular layer of rat cerebellum.

Graphical abstract: Laser capture microdissection and native mass spectrometry for spatially-resolved analysis of intact protein assemblies in tissue

Supplementary files

Article information

Article type
Edge Article
Submitted
19 sep 2023
Accepted
03 mar 2024
First published
07 mar 2024
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2024,15, 5723-5729

Laser capture microdissection and native mass spectrometry for spatially-resolved analysis of intact protein assemblies in tissue

James W. Hughes, E. K. Sisley, O. J. Hale and H. J. Cooper, Chem. Sci., 2024, 15, 5723 DOI: 10.1039/D3SC04933G

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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