Issue 32, 2024

Oxygen-tolerant, eosin Y mediated synthesis of protein–polymer biohybrids and protein-coated polymer nanoparticles

Abstract

To avoid metal catalysts that are commonly used in conventional approaches for the synthesis of protein–polymer conjugates, an eosin Y/TEMED mediated, photoinduced polymerization of vinyl monomers was optimized. This oxygen tolerant, photoinduced approach allowed the grafting of a series of hydrophobic, hydrophilic and responsive polymers with quantitative protein macroinitiator consumption. CALB bioconjugates were also synthesized and found to retain part of the parent protein activity for extended periods of time. Notably, when BSA was used in the absence of an initiator, protein-coated nanoparticles were shown to form during emulsion polymerization.

Graphical abstract: Oxygen-tolerant, eosin Y mediated synthesis of protein–polymer biohybrids and protein-coated polymer nanoparticles

Supplementary files

Article information

Article type
Paper
Submitted
14 apr 2024
Accepted
08 jul 2024
First published
10 jul 2024
This article is Open Access
Creative Commons BY-NC license

Polym. Chem., 2024,15, 3246-3255

Oxygen-tolerant, eosin Y mediated synthesis of protein–polymer biohybrids and protein-coated polymer nanoparticles

E. Voutyritsa, T. Lazou, J. Bushi, S. Margaritaki, M. Charitaki, S. M. Christensen, N. S. Hatzakis and K. Velonia, Polym. Chem., 2024, 15, 3246 DOI: 10.1039/D4PY00407H

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