Issue 5, 2024

Functional magnetic nanoparticles for protein delivery applications: understanding protein–nanoparticle interactions

Abstract

Iron oxide nanoparticles (IONPs) surface functionalised with thermo-responsive polymers can encapsulate therapeutic proteins and release them upon heating with an alternating magnetic field above the lower critical solution temperature (LCST). In order to make this delivery system clinically-relevant, we prepared IONPs coated with poly-N-isopropylmethacrylamide (PNIPMAM), a polymer with LCST above human body temperature. The optimal polymer chain length and nanoparticle size to achieve LCST of ca. 45 °C were 19 kDa PNIPMAM and 16 nm IONPs. The PNIPMAM-coated IONPs could encapsulate a range of proteins which were released upon heating above LCST in the presence of a competitor protein or serum. A small amount of encapsulated protein leakage was observed below LCST. The efficiency of protein encapsulation and release was correlated with molecular weight and glycosylation state of the proteins. Magnetic heating resulted in a faster protein release as compared to conventional heating without significant temperature increase of the bulk solution.

Graphical abstract: Functional magnetic nanoparticles for protein delivery applications: understanding protein–nanoparticle interactions

Supplementary files

Article information

Article type
Paper
Submitted
08 sep 2023
Accepted
20 des 2023
First published
29 des 2023
This article is Open Access
Creative Commons BY-NC license

Nanoscale, 2024,16, 2466-2477

Functional magnetic nanoparticles for protein delivery applications: understanding protein–nanoparticle interactions

R. Sharma, D. Ungar, E. Dyson, S. Rimmer and V. Chechik, Nanoscale, 2024, 16, 2466 DOI: 10.1039/D3NR04544G

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