Issue 20, 2024

Recent research on material-based methods for isolation of extracellular vesicles

Abstract

Extracellular vesicles (EVs) are nanoparticles secreted by cells with a closed phospholipid bilayer structure, which can participate in various physiological and pathological processes and have significant clinical value in disease diagnosis, targeted therapy and prognosis assessment. EV isolation methods currently include differential ultracentrifugation, ultrafiltration, size exclusion chromatography, immunoaffinity, polymer co-precipitation and microfluidics. In addition, material-based biochemical or biophysical approaches relying on intrinsic properties of the material or its surface-modified functionalized monomers, demonstrated unique advantages in the efficient isolation of EVs. In order to provide new ideas for the subsequent development of material-based EV isolation methods, this review will focus on the principle, research status and application prospects of material-based EV isolation methods based on different material carriers and functional monomers.

Graphical abstract: Recent research on material-based methods for isolation of extracellular vesicles

Supplementary files

Article information

Article type
Critical Review
Submitted
28 feb 2024
Accepted
25 apr 2024
First published
26 apr 2024

Anal. Methods, 2024,16, 3179-3191

Recent research on material-based methods for isolation of extracellular vesicles

M. Chen, J. Li, Y. Lin, X. Li, Y. Yu, S. Zhou, F. Xu, Q. Zhang, H. Zhang and W. Wang, Anal. Methods, 2024, 16, 3179 DOI: 10.1039/D4AY00370E

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