Issue 8, 2024

Isothermal amplification-based microfluidic devices for detecting foodborne pathogens: a review

Abstract

The gold standard for nucleic acid amplification-based diagnosis is the polymerase chain reaction (PCR). The PCR recognizes the targets such as foodborne pathogens by amplifying their specific genes. The integration of nucleic acid amplification-based assays on microfluidic platforms represents a highly promising solution for convenient, cheap, and effective control of foodborne pathogens. However, the application of the PCR is limited to on-site detection because the method requires sophisticated equipment for temperature control, which makes it complicated for microfluidic integration. Alternatively, isothermal amplification methods are promising tools for integrating microfluidic platforms for on-site detection of foodborne pathogens. This review summarized advances in isothermal amplification-based microfluidic devices for detecting foodborne pathogens. Different nucleic acid extraction approaches and the integration of these approaches in microfluidic platforms were first reviewed. Microfluidic platforms integrated with three common isothermal amplification methods including loop-mediated isothermal amplification, recombinase polymerase amplification, and recombinase-aided amplification were then described and discussed.

Graphical abstract: Isothermal amplification-based microfluidic devices for detecting foodborne pathogens: a review

Article information

Article type
Critical Review
Submitted
16 nov 2023
Accepted
19 jan 2024
First published
19 jan 2024

Anal. Methods, 2024,16, 1150-1157

Isothermal amplification-based microfluidic devices for detecting foodborne pathogens: a review

T. N. D. Trinh and N. N. Nam, Anal. Methods, 2024, 16, 1150 DOI: 10.1039/D3AY02039H

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