Issue 43, 2023

Impact of library input on the hit discovery rate in DNA-encoded chemical library selections

Abstract

DNA-encoded chemical libraries (DELs) are powerful drug discovery tools, enabling the parallel screening of millions of DNA-barcoded compounds. We investigated how the DEL input affects the hit discovery rate in DEL screenings. Evaluation of selection fingerprints revealed that the use of approximately 105 copies of each library member is required for the confident identification of nanomolar hits, using generally applicable methodologies.

Graphical abstract: Impact of library input on the hit discovery rate in DNA-encoded chemical library selections

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Article information

Article type
Edge Article
Submitted
17 jul 2023
Accepted
29 sep 2023
First published
17 okt 2023
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2023,14, 12026-12033

Impact of library input on the hit discovery rate in DNA-encoded chemical library selections

S. Puglioli, S. Oehler, L. Prati, J. Scheuermann, G. Bassi, S. Cazzamalli, D. Neri and N. Favalli, Chem. Sci., 2023, 14, 12026 DOI: 10.1039/D3SC03688J

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