Issue 6, 2023

Recent progress in nucleic acid detection with CRISPR

Abstract

Recent advances in CRISPR-based biotechnologies have greatly expanded our capabilities to repurpose CRISPR for the development of molecular diagnostic systems. The key attribute that allows CRISPR to be widely utilized is its programmable and highly specific nature. In this review, we first illustrate the principle of the class 2 CRISPR nucleases for molecular diagnostics which originates from their immunologic defence systems. Next, we present the CRISPR-based schemes in the application of diagnostics with amplification-assisted or amplification-free strategies. By highlighting some of the recent advances we interpret how general bioengineering methodologies can be integrated with CRISPR. Finally, we discuss the challenges and exciting prospects for future CRISPR-based biosensing development. We hope that this review will guide the reader to systematically learn the start-of-the-art development of CRISPR-mediated nucleic acid detection and understand how to apply the CRISPR nucleases with different design concepts to more general applications in diagnostics and beyond.

Graphical abstract: Recent progress in nucleic acid detection with CRISPR

Article information

Article type
Critical Review
Submitted
04 okt 2022
Accepted
20 jan 2023
First published
24 jan 2023

Lab Chip, 2023,23, 1467-1492

Recent progress in nucleic acid detection with CRISPR

F. X. Liu, J. Q. Cui, Z. Wu and S. Yao, Lab Chip, 2023, 23, 1467 DOI: 10.1039/D2LC00928E

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