Issue 39, 2023

Sensitive quantification of short-chain fatty acids combined with global metabolomics in microbiome cultures

Abstract

The microbiome has been identified to have a key role for the physiology of their human host. One of the major impacts is the clearance of bacterial pathogens. We have now developed a chemoselective probe methodology for the absolute quantification of short-chain fatty acids at low nM concentrations, with high reproducibility and spiked isotope labelled internal standards. Immobilization to magnetic beads allows for separation from the matrix and the tagged metabolites upon bioorthogonal cleavage can be analyzed via UHPLC-MS. The major advantage of our sensitive method is the simple combination with global metabolomics analysis as only a small sample volume is required. We have applied this chemical metabolomics strategy for targeted SCFA analysis combined with global metabolomics on gut microbiome co-cultures with Salmonella and investigated the effect of antibiotic treatment.

Graphical abstract: Sensitive quantification of short-chain fatty acids combined with global metabolomics in microbiome cultures

Supplementary files

Article information

Article type
Communication
Submitted
11 mar 2023
Accepted
19 apr 2023
First published
19 apr 2023
This article is Open Access
Creative Commons BY license

Chem. Commun., 2023,59, 5843-5846

Sensitive quantification of short-chain fatty acids combined with global metabolomics in microbiome cultures

W. Lin, F. R. García, E. L. Norin, D. Kart, L. Engstrand, J. Du and D. Globisch, Chem. Commun., 2023, 59, 5843 DOI: 10.1039/D3CC01223A

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