Issue 8, 2023

The effect of metalation on antimicrobial piscidins imbedded in normal and oxidized lipid bilayers

Abstract

Metalation of the N-terminal Amino Terminal Cu(II)- and Ni(II)-binding (ATCUN) motif may enhance the antimicrobial properties of piscidins. Molecular dynamics simulations of free and nickelated piscidins 1 and 3 (P1 and P3) were performed in 3 : 1 POPC/POPG and 2.6 : 1 : 0.4 POPC/POPG/aldo-PC bilayers (POPC, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine: POPG, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol; aldo-PC, 1-palmitoyl-2-(9′-oxo-nonanoyl)-sn-glycero-3-phosphocholine) bilayer models. Nickel(II) binding decreases the conformation dynamics of the ATCUN motif and lowers the charge of the N-terminus to allow it to embed deeper in the bilayer without significantly changing the overall depth due to interactions of the charged half-helix of the peptide with the headgroups. Phe1⋯Ni2+ cation–π and Phe2–Phe1 CH–π interactions contribute to a small fraction of structures within the nickelated P1 simulations and may partially protect a bound metal from metal-centered chemical activity. The substitution of Phe2 for Ile2 in P3 sterically blocks conformations with cation–π interactions offering less protection to the metal. This difference between metalated P1 and P3 may indicate a mechanism by which peptide sequence can influence antimicrobial properties. Any loss of bilayer integrity due to chain reversal of the oxidized phospholipid chains of aldo-PC may be enhanced in the presence of metalated piscidins.

Graphical abstract: The effect of metalation on antimicrobial piscidins imbedded in normal and oxidized lipid bilayers

Supplementary files

Article information

Article type
Paper
Submitted
17 mar 2023
Accepted
02 jun 2023
First published
07 jun 2023
This article is Open Access
Creative Commons BY license

RSC Chem. Biol., 2023,4, 573-586

The effect of metalation on antimicrobial piscidins imbedded in normal and oxidized lipid bilayers

A. Dreab and C. A. Bayse, RSC Chem. Biol., 2023, 4, 573 DOI: 10.1039/D3CB00035D

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