Issue 13, 2023

Exploration of utility of combined optical photothermal infrared and Raman imaging for investigating the chemical composition of microcalcifications in breast cancer

Abstract

Microcalcifications play an important role in cancer detection. They are evaluated by their radiological and histological characteristics but it is challenging to find a link between their morphology, their composition and the nature of a specific type of breast lesion. Whilst there are some mammographic features that are either typically benign or typically malignant often the appearances are indeterminate. Here, we explore a large range of vibrational spectroscopic and multiphoton imaging techniques in order to gain more information about the composition of the microcalcifications. For the first time, we validated the presence of carbonate ions in the microcalcifications by O-PTIR and Raman spectroscopy at the same time, the same location and the same high resolution (0.5 μm). Furthermore, the use of multiphoton imaging allowed us to create stimulated Raman histology (SRH) images which mimic histological images with all chemical information. In conclusion, we established a protocol for efficiently analysing the microcalcifications by iteratively refining the area of interest.

Graphical abstract: Exploration of utility of combined optical photothermal infrared and Raman imaging for investigating the chemical composition of microcalcifications in breast cancer

Article information

Article type
Paper
Submitted
26 jul 2022
Accepted
21 feb 2023
First published
21 feb 2023
This article is Open Access
Creative Commons BY license

Anal. Methods, 2023,15, 1620-1630

Exploration of utility of combined optical photothermal infrared and Raman imaging for investigating the chemical composition of microcalcifications in breast cancer

P. Bouzy, I. D. Lyburn, S. E. Pinder, R. Scott, J. Mansfield, J. Moger, C. Greenwood, I. Bouybayoune, E. Cornford, K. Rogers and N. Stone, Anal. Methods, 2023, 15, 1620 DOI: 10.1039/D2AY01197B

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