Issue 41, 2022

Parallel DNA circuits by autocatalytic strand displacement and nanopore readout

Abstract

DNA nanotechnology provides a unique opportunity for molecular computation, with strand displacement reactions enabling controllable reorganization of nanostructures. Additional DNA strand exchange strategies with high selectivity for input will enable novel complex systems including biosensing applications. Herein, we propose an autocatalytic strand displacement (ACSD) circuit: initiated by DNA breathing and accelerated by a seesaw catalytic reaction, ACSD ensures that only the correct base sequence starts the catalytic cycle. Analogous to an electronic circuit with a variable resistor, two ACSD reactions with different rates are connected in parallel to mimic a parallel circuit containing branches with different resistances. Finally, we introduce a multiplexed nanopore sensing platform to report the output results of a parallel path selection system at the single-molecule level. By combining the ACSD strategy with fast and sensitive single-molecule nanopore readout, a new generation of DNA-based computing tools is established.

Graphical abstract: Parallel DNA circuits by autocatalytic strand displacement and nanopore readout

Supplementary files

Article information

Article type
Paper
Submitted
22 jul 2022
Accepted
07 okt 2022
First published
07 okt 2022
This article is Open Access
Creative Commons BY license

Nanoscale, 2022,14, 15507-15515

Parallel DNA circuits by autocatalytic strand displacement and nanopore readout

J. Zhu, J. Kong, U. F. Keyser and E. Wang, Nanoscale, 2022, 14, 15507 DOI: 10.1039/D2NR04048D

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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