Issue 14, 2022

Surface enhanced Raman scattering for probing cellular biochemistry

Abstract

Surface enhanced Raman scattering (SERS) from biomolecules in living cells enables the sensitive, but also very selective, probing of their biochemical composition. This minireview discusses the developments of SERS probing in cells over the past years from the proof-of-principle to observe a biochemical status to the characterization of molecule–nanostructure and molecule–molecule interactions and cellular processes that involve a wide variety of biomolecules and cellular compartments. Progress in applying SERS as a bioanalytical tool in living cells, to gain a better understanding of cellular physiology and to harness the selectivity of SERS, has been achieved by a combination of live cell SERS with several different approaches. They range from organelle targeting, spectroscopy of relevant molecular models, and the optimization of plasmonic nanostructures to the application of machine learning and help us to unify the information from defined biomolecules and from the cell as an extremely complex system.

Graphical abstract: Surface enhanced Raman scattering for probing cellular biochemistry

Article information

Article type
Minireview
Submitted
23 jan 2022
Accepted
15 mar 2022
First published
15 mar 2022
This article is Open Access
Creative Commons BY-NC license

Nanoscale, 2022,14, 5314-5328

Surface enhanced Raman scattering for probing cellular biochemistry

C. Spedalieri and J. Kneipp, Nanoscale, 2022, 14, 5314 DOI: 10.1039/D2NR00449F

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