Issue 7, 2022

Polyclonal aptamer libraries as binding entities on a graphene FET based biosensor for the discrimination of apo- and holo-retinol binding protein 4

Abstract

Oligonucleotide DNA aptamers represent an emergently important class of binding entities towards as different analytes as small molecules or even whole cells. Without requiring the canonical isolation of individual aptamers following the SELEX process, the focused polyclonal libraries prepared by this in vitro evolution and selection can directly be used to label their dedicated targets and to serve as binding molecules on surfaces. Here we report the first instance of a sensor able to discriminate between loaded and unloaded retinol-binding protein 4 (RBP4), an important biomarker for the prediction of diabetes and kidney disease. The sensor relies on two aptamer libraries tuned such that they discriminate between the protein isoforms, requiring no further sample labelling to detect RBP4 in both states. The evolution, binding properties of the libraries and the functionalization of graphene FET sensor chips are presented as well as the functionality of the resulting biosensor.

Graphical abstract: Polyclonal aptamer libraries as binding entities on a graphene FET based biosensor for the discrimination of apo- and holo-retinol binding protein 4

Supplementary files

Article information

Article type
Communication
Submitted
17 nov 2021
Accepted
28 apr 2022
First published
29 apr 2022

Nanoscale Horiz., 2022,7, 770-778

Polyclonal aptamer libraries as binding entities on a graphene FET based biosensor for the discrimination of apo- and holo-retinol binding protein 4

A. Kissmann, J. Andersson, A. Bozdogan, V. Amann, M. Krämer, H. Xing, H. F. Raber, D. H. Kubiczek, P. Aspermair, W. Knoll and F. Rosenau, Nanoscale Horiz., 2022, 7, 770 DOI: 10.1039/D1NH00605C

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