Issue 27, 2021

Employing defined bioconjugates to generate chemically functionalised gold nanoparticles for in vitro diagnostic applications

Abstract

Novel methods for introducing chemical and biological functionality to the surface of gold nanoparticles serve to increase the utility of this class of nanomaterials across a range of applications. To date, methods for functionalising gold surfaces have relied upon uncontrollable non-specific adsorption, bespoke chemical linkers, or non-generalisable protein–protein interactions. Herein we report a versatile method for introducing functionality to gold nanoparticles by exploiting the strong interaction between chemically functionalised bovine serum albumin (f-BSA) and citrate-capped gold nanoparticles (AuNPs). We establish the generalisability of the method by introducing a variety of functionalities to gold nanoparticles using cheap, commercially available chemical linkers. The utility of this approach is further demonstrated through the conjugation of the monoclonal antibody Ontruzant to f-BSA–AuNPs using inverse electron-demand Diels–Alder (iEDDA) click chemistry, a hitherto unexplored chemistry for AuNP–IgG conjugation. Finally, we show that the AuNP–Ontruzant particles generated via f-BSA–AuNPs have a greater affinity for their target in a lateral flow format when compared to conventional physisorption, highlighting the potential of this technology for producing sensitive diagnostic tests.

Graphical abstract: Employing defined bioconjugates to generate chemically functionalised gold nanoparticles for in vitro diagnostic applications

Supplementary files

Article information

Article type
Paper
Submitted
23 apr 2021
Accepted
22 jun 2021
First published
22 jun 2021
This article is Open Access
Creative Commons BY license

Nanoscale, 2021,13, 11921-11931

Employing defined bioconjugates to generate chemically functionalised gold nanoparticles for in vitro diagnostic applications

D. A. Richards, M. R. Thomas, P. A. Szijj, J. Foote, Y. Chen, J. C. F. Nogueira, V. Chudasama and M. M. Stevens, Nanoscale, 2021, 13, 11921 DOI: 10.1039/D1NR02584H

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements