Issue 8, 2021

A novel electrochemical lung cancer biomarker cytokeratin 19 fragment antigen 21-1 immunosensor based on Si3N4/MoS2 incorporated MWCNTs and core–shell type magnetic nanoparticles

Abstract

Lung cancer is one of deadliest and most life threatening cancer types. Cytokeratin 19 fragment antigen 21-1 (CYFRA 21-1) is a significant biomarker for the diagnosis of non-small cell lung cancer (NSCLC). Due to these reasons, a novel electrochemical immunosensor based on a silicon nitride (Si3N4)–molybdenum disulfide (MoS2) composite on multi-walled carbon nanotubes (Si3N4/MoS2–MWCNTs) as an electrochemical sensor platform and core–shell type magnetic mesoporous silica nanoparticles@gold nanoparticles (MMSNs@AuNPs) as a signal amplifier was presented for CYFRA21-1 detection in this study. Capture antibody (Ab1) immobilization on a Si3N4/MoS2–MWCNT modified glassy carbon electrode (Si3N4/MoS2–MWCNTs/GCE) was firstly successfully performed by stable electrostatic/ionic interactions between the –NH2 groups of the capture antibody and the polar groups of Si3N4/MoS2. Then, specific antibody–antigen interactions between the electrochemical sensor platform and the signal amplifier formed a novel voltammetric CYFRA21-1 immunosensor. The prepared composite materials and electrochemical sensor surfaces were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). A linearity range of 0.01–1.0 pg mL−1 and a low detection limit (LOD) of 2.00 fg mL−1 were also obtained for analytical applications. Thus, the proposed immunosensor based on Si3N4/MoS2–MWCNTs and MMSNs@AuNPs has great potential for medical diagnosis of lung cancer.

Graphical abstract: A novel electrochemical lung cancer biomarker cytokeratin 19 fragment antigen 21-1 immunosensor based on Si3N4/MoS2 incorporated MWCNTs and core–shell type magnetic nanoparticles

Supplementary files

Article information

Article type
Paper
Submitted
13 jan 2021
Accepted
02 feb 2021
First published
02 feb 2021

Nanoscale, 2021,13, 4660-4669

A novel electrochemical lung cancer biomarker cytokeratin 19 fragment antigen 21-1 immunosensor based on Si3N4/MoS2 incorporated MWCNTs and core–shell type magnetic nanoparticles

M. L. Yola, N. Atar and N. Özcan, Nanoscale, 2021, 13, 4660 DOI: 10.1039/D1NR00244A

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