Issue 23, 2021

A microfluidic chip with a serpentine channel enabling high-throughput cell separation using surface acoustic waves

Abstract

As an acute inflammatory response, sepsis may cause septic shock and multiple organ failure. Rapid and reliable detection of pathogens from blood samples can promote early diagnosis and treatment of sepsis. However, traditional pathogen detection methods rely on bacterial blood culture, which is complex and time-consuming. Although pre-separation of bacteria from blood can help with the identification of pathogens for diagnosis, the required low-velocity fluid environment of most separation techniques greatly limits the processing capacity for blood samples. Here, we present an acoustofluidic device for high-throughput bacterial separation from human blood cells. Our device utilizes a serpentine microfluidic design and standing surface acoustic waves (SSAWs), and separates bacteria from blood cells effectively based on their size difference. The serpentine microstructure allows the operating distance of the acoustic field to be multiplied in a limited chip size via the “spatial multiplexing” and “pressure node matching” of SSAW field. Microscopic observation and flow cytometry analysis shows that the device is helpful in improving the flow rate (2.6 μL min−1 for blood samples; the corresponding velocity is ∼3 cm s−1) without losing separation purity or cell recovery. The serpentine microfluidic design provides a compatible solution for high-throughput separation, which can synergize with other functional designs to improve device performance. Further, its advantages such as low cost, high biocompatibility, label-free separation and ability to integrate with on-chip biosensors are promising for clinical utility in point-of-care diagnostic platforms.

Graphical abstract: A microfluidic chip with a serpentine channel enabling high-throughput cell separation using surface acoustic waves

Supplementary files

Article information

Article type
Paper
Submitted
17 sep 2021
Accepted
29 okt 2021
First published
01 nov 2021

Lab Chip, 2021,21, 4608-4617

A microfluidic chip with a serpentine channel enabling high-throughput cell separation using surface acoustic waves

S. Ning, S. Liu, Y. Xiao, G. Zhang, W. Cui and M. Reed, Lab Chip, 2021, 21, 4608 DOI: 10.1039/D1LC00840D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements