Issue 23, 2021

Optofluidic ptychography on a chip

Abstract

We report the implementation of a fully on-chip, lensless microscopy technique termed optofluidic ptychography. This imaging modality complements the miniaturization provided by microfluidics and allows the integration of ptychographic microscopy into various lab-on-a-chip devices. In our prototype, we place a microfluidic channel on the top surface of a coverslip and coat the bottom surface with a scattering layer. The channel and the coated coverslip substrate are then placed on top of an image sensor for diffraction data acquisition. Similar to the operation of a flow cytometer, the device utilizes microfluidic flow to deliver specimens across the channel. The diffracted light from the flowing objects is modulated by the scattering layer and recorded by the image sensor for ptychographic reconstruction, where high-resolution quantitative complex images are recovered from the diffraction measurements. By using an image sensor with a 1.85 μm pixel size, our device can resolve the 550 nm linewidth on the resolution target. We validate the device by imaging different types of biospecimens, including C. elegans, yeast cells, paramecium, and closterium sp. We also demonstrate a high-resolution ptychographic reconstruction at a video framerate of 30 frames per second. The reported technique can address a wide range of biomedical needs and engenders new ptychographic imaging innovations in a flow cytometer configuration.

Graphical abstract: Optofluidic ptychography on a chip

Supplementary files

Article information

Article type
Communication
Submitted
16 aug 2021
Accepted
19 okt 2021
First published
20 okt 2021

Lab Chip, 2021,21, 4549-4556

Author version available

Optofluidic ptychography on a chip

P. Song, C. Guo, S. Jiang, T. Wang, P. Hu, D. Hu, Z. Zhang, B. Feng and G. Zheng, Lab Chip, 2021, 21, 4549 DOI: 10.1039/D1LC00719J

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