Issue 23, 2021

One-pot biocatalytic synthesis of nylon monomers from cyclohexanol using Escherichia coli-based concurrent cascade consortia

Abstract

We developed a multienzyme biocatalytic cascade with high atom efficiency and a self-sufficient redox network for the synthesis of nylon monomers without adding auxiliary enzymes to recycle cofactors. Herein, a cell-based modularization strategy was applied to produce 6-aminohexanoic acid and ε-caprolactam from cyclohexanol. The designed consortia of E. coli containing the respective modules was found to produce >97% of 6-aminohexanoic acid (from 100 mM cyclohexanol) and 28% of caprolactam (from 10 mM cyclohexanol) with 2-fold excess of propylamine in a one-pot reaction. The use of cheaper substrates and co-substrates makes it an economically beneficial approach for the production of nylon precursors.

Graphical abstract: One-pot biocatalytic synthesis of nylon monomers from cyclohexanol using Escherichia coli-based concurrent cascade consortia

Supplementary files

Article information

Article type
Communication
Submitted
23 aug 2021
Accepted
04 nov 2021
First published
04 nov 2021

Green Chem., 2021,23, 9447-9453

One-pot biocatalytic synthesis of nylon monomers from cyclohexanol using Escherichia coli-based concurrent cascade consortia

S. Sarak, T. P. Khobragade, H. Jeon, A. D. Pagar, P. Giri, S. Lee and H. Yun, Green Chem., 2021, 23, 9447 DOI: 10.1039/D1GC03056F

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements