Issue 21, 2021

Phenylboronic acid-functionalized polyaminoglycoside as an effective CRISPR/Cas9 delivery system

Abstract

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) gene editing technology is a promising approach for cancer therapy, and its application practice urgently requires a safe and effective gene carrier. In this work, we focus on the design of a phenylboronic acid (PBA)-functionalized, disulfide bonded branched polyaminoglycoside (SS-HPT-P) as a robust delivery vector of the CRISPR-Cas9 system. SS-HPT-P showed great tumor-targeting performance, reduction-responsive degradability, and gene transfection ability. The typical pCas9-surv (one CRISPR-Cas9 plasmid that targets and knocks out the survivin gene) delivery mediated by SS-HPT-P exhibited gene editing performance in the A549 cell line, confirming the feasibility of SS-HPT-P to effectively deliver the CRISPR-Cas9 system. SS-HPT-P/pCas9-surv could effectively inhibit the proliferation of tumor cells both in vitro and in vivo, suggesting the potential of PBA-functionalized nanocarriers for cancer gene therapy. The present work provides a promising approach for the treatment of malignant tumors.

Graphical abstract: Phenylboronic acid-functionalized polyaminoglycoside as an effective CRISPR/Cas9 delivery system

Supplementary files

Article information

Article type
Paper
Submitted
01 feb 2021
Accepted
27 feb 2021
First published
03 mar 2021

Biomater. Sci., 2021,9, 7104-7114

Phenylboronic acid-functionalized polyaminoglycoside as an effective CRISPR/Cas9 delivery system

M. Shao, Y. Qi, D. Sui and F. Xu, Biomater. Sci., 2021, 9, 7104 DOI: 10.1039/D1BM00185J

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