Issue 2, 2019

A lithium-ion-active aerolysin nanopore for effectively trapping long single-stranded DNA

Abstract

Wild-type aerolysin (AeL) nanopores allow direct single nucleotide discrimination of very short oligonucleotides (≤10 nt) without labelling, which shows great potential for DNA sensing. To achieve real applications, one major obstacle of AeL is its poor capture ability of long single-stranded DNA (ssDNA, >10 nt). Here, we have proposed a novel and robust strategy for the electrostatic focusing of long ssDNA into a lithium-chloride (LiCl)-active AeL. By using this method, for the first time we have demonstrated AeL detection of ssDNA longer than 100 nt. Due to screening more negative charges, LiCl improves AeL capture ability of long ssDNA (i.e. 60 nt) by 2.63- to 10.23-fold compared to KCl. Further calculations and molecular dynamics simulations revealed that strong binding between Li+ and the negatively charged residue neutralized the AeL, leading to a reduction in the energy barrier for ssDNA capture. These findings facilitate the future high-throughput applications of AeL in genetic and epigenetic diagnostics.

Graphical abstract: A lithium-ion-active aerolysin nanopore for effectively trapping long single-stranded DNA

Supplementary files

Article information

Article type
Edge Article
Submitted
03 sep 2018
Accepted
12 nov 2018
First published
13 nov 2018
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2019,10, 354-358

A lithium-ion-active aerolysin nanopore for effectively trapping long single-stranded DNA

Z. Hu, M. Li, S. Liu, Y. Ying and Y. Long, Chem. Sci., 2019, 10, 354 DOI: 10.1039/C8SC03927E

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