Volume 219, 2019

A ‘catch-and-release’ receptor for the cholera toxin

Abstract

Stimuli-responsive receptors for the recognition unit of the cholera toxin (CTB) have been prepared by attaching multiple copies of its natural carbohydrate ligand, the GM1 oligosaccharide, to a thermoresponsive polymer scaffold. Below their lower critical solution temperature (LCST), polymers complex CTB with nanomolar affinity. When heated above their LCST, polymers undergo a reversible coil to globule transition which renders a proportion of the carbohydrate recognition motifs inaccessible to CTB. This thermally-modulated decrease in the avidity of the material for the protein has been used to reversibly capture CTB from solution, enabling its convenient isolation from a complex mixture.

Graphical abstract: A ‘catch-and-release’ receptor for the cholera toxin

Associated articles

Supplementary files

Article information

Article type
Paper
Submitted
11 feb 2019
Accepted
20 mar 2019
First published
21 mar 2019

Faraday Discuss., 2019,219, 112-127

A ‘catch-and-release’ receptor for the cholera toxin

C. S. Mahon, G. C. Wildsmith, D. Haksar, E. de Poel, J. M. Beekman, R. J. Pieters, M. E. Webb and W. B. Turnbull, Faraday Discuss., 2019, 219, 112 DOI: 10.1039/C9FD00017H

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