Issue 11, 2018

A novel porphyrin-based near-infrared fluorescent probe for hypochlorite detection and its application in vitro and in vivo

Abstract

Reactive oxygen species (ROS), especially HOCl/ClO, have been demonstrated to play essential roles in both physiological and pathological processes, and an abnormal level of HOCl/ClO is related to some diseases. In this work, a very fast responsive (within 30 seconds) porphyrin-based fluorescent probe, TPP-TCF, for ClO with a NIR emissive wavelength was prepared. This probe exhibited excellent selectivity towards ClO and would not be interfered with by other ROS and typical nucleophiles. The limit of detection (LOD) for ClO was evaluated to be 0.29 μM, indicating high sensitivity towards ClO. In further bioimaging experiments, TPP-TCF displayed low-cytotoxicity and good cell penetrability for recognizing exogenous ClO in HeLa cells. Moreover, this probe was successfully applied in imaging endogenous ClO in living animals.

Graphical abstract: A novel porphyrin-based near-infrared fluorescent probe for hypochlorite detection and its application in vitro and in vivo

Supplementary files

Article information

Article type
Paper
Submitted
28 mar 2018
Accepted
18 apr 2018
First published
19 apr 2018

Analyst, 2018,143, 2641-2647

A novel porphyrin-based near-infrared fluorescent probe for hypochlorite detection and its application in vitro and in vivo

X. Wang, J. Min, W. Wang, Y. Wang, G. Yin and R. Wang, Analyst, 2018, 143, 2641 DOI: 10.1039/C8AN00586A

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