Volume 205, 2017

Real-time dynamic SERS detection of galectin using glycan-decorated gold nanoparticles

Abstract

We present the application of surface-enhanced Raman scattering (SERS) spectroscopy for the fast, sensitive and highly specific detection of the galectin-9 (Gal-9) protein in binding buffer (mimicking natural conditions). The method involves the use of specifically designed nanotags comprising glycan-decorated gold nanoparticles encoded with 4-mercaptobenzoic acid. At fast time scales Gal-9 can be detected down to a concentration of 1.2 nM by monitoring the SERS signal of the reporter, driven by aggregation of the functionalized Au NPs tags, induced by Gal-9 recognition. We additionally demonstrate that the sensitivity and concentration working range of the sensor can be tuned via control of aggregation dynamics and cluster size distribution.

Associated articles

Supplementary files

Article information

Article type
Paper
Submitted
07 apr 2017
Accepted
04 mai 2017
First published
04 mai 2017

Faraday Discuss., 2017,205, 363-375

Real-time dynamic SERS detection of galectin using glycan-decorated gold nanoparticles

J. Langer, I. García and L. M. Liz-Marzán, Faraday Discuss., 2017, 205, 363 DOI: 10.1039/C7FD00123A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements