Issue 21, 2016

Recognition of tumor cells by immuno-SERS-markers in a microfluidic chip at continuous flow

Abstract

SERS active nanoparticles were labeled with a reporter molecule and conjugated with anti-EpCAM antibodies. These immuno SERS markers were mixed with leukocytes, MCF-7 breast cancer cells and polystyrene beads, and the mixture was injected into a microfluidic quartz chip. Raman spectra were acquired at 785 nm excitation with 25 milliseconds exposure time in a continuous flow regime. Spectral unmixing by N-FINDR identified spectral signatures of SERS-labelled cells and polystyrene beads. This approach demonstrated rapid and reproducible SERS-assisted cell detection. Strategies are discussed to further increase the throughput for cell sorting.

Graphical abstract: Recognition of tumor cells by immuno-SERS-markers in a microfluidic chip at continuous flow

Article information

Article type
Communication
Submitted
01 aug 2016
Accepted
27 sep 2016
First published
27 sep 2016

Analyst, 2016,141, 5986-5989

Recognition of tumor cells by immuno-SERS-markers in a microfluidic chip at continuous flow

I. Freitag, C. Beleites, S. Dochow, J. H. Clement, C. Krafft and J. Popp, Analyst, 2016, 141, 5986 DOI: 10.1039/C6AN01739H

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