Issue 25, 2015

Collagen labelling with an azide-proline chemical reporter in live cells

Abstract

We have developed a strategy for selective imaging of collagen in live foetal ovine osteoblasts. Our approach involves the incorporation of an azide-tagged proline in the biosynthesis of collagen followed by labelling using a strain-promoted [3+2] azide–alkyne cycloaddition reaction.

Graphical abstract: Collagen labelling with an azide-proline chemical reporter in live cells

Supplementary files

Article information

Article type
Communication
Submitted
08 okt 2014
Accepted
17 nov 2014
First published
18 nov 2014
This article is Open Access
Creative Commons BY license

Chem. Commun., 2015,51, 5250-5252

Author version available

Collagen labelling with an azide-proline chemical reporter in live cells

B. Amgarten, R. Rajan, N. Martínez-Sáez, B. L. Oliveira, I. S. Albuquerque, R. A. Brooks, D. G. Reid, M. J. Duer and G. J. L. Bernardes, Chem. Commun., 2015, 51, 5250 DOI: 10.1039/C4CC07974D

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