Issue 8, 2024

Sugar alcohol-modified polyester nanoparticles for gene delivery via selective caveolae-mediated endocytosis

Abstract

Nucleic acid-based drugs are changing the scope of emerging medicine in preventing and treating diseases. Nanoparticle systems based on lipids and polymers developed to navigate tissue-level and cellular-level barriers are now emerging as vector systems that can be translated to clinical settings. A class of polymers, poly(β-amino esters) (PBAEs) known for their chemical flexibility and biodegradability, has been explored for gene delivery. These polymers are sensitive to changes in the monomer composition affecting transfection efficiency. Hence to add functionality to these polymers, we partially substituted ligands to an identified effective polymer chemistry. We report here a new series of statistical copolymers based on PBAEs where the backbone is modified with sugar alcohols to selectively facilitate the caveolae-mediated endocytosis pathway of cellular transport. These ligands are grafted at the polymer's backbone, thereby establishing a new strategy of modification in PBAEs. We demonstrate that these polymers form nanoparticles with DNA, show effective complexation and cargo release, enter the cell via selective caveolae-mediated endocytosis, exhibit low cytotoxicity, and increase transfection in neuronal cells.

Graphical abstract: Sugar alcohol-modified polyester nanoparticles for gene delivery via selective caveolae-mediated endocytosis

Supplementary files

Article information

Article type
Paper
Submitted
20 okt 2023
Accepted
28 jan 2024
First published
30 jan 2024

Nanoscale, 2024,16, 4114-4124

Sugar alcohol-modified polyester nanoparticles for gene delivery via selective caveolae-mediated endocytosis

B. Reshma G, C. Miglani, A. Pal and M. Ganguli, Nanoscale, 2024, 16, 4114 DOI: 10.1039/D3NR05300H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements