Issue 13, 2024

Electroactive polymer tag modified nanosensors for enhanced intracellular ATP detection

Abstract

ATP plays a crucial role in cell energy supply, so the quantification of intracellular ATP levels is particularly important for understanding many physio-pathological processes. The intracellular quantification of this non-electroactive molecule can be realized using aptamer-modified nanoelectrodes, but is hindered by the limited quantity of modification and electroactive tags on the nanosized electrodes. Herein, we developed a simple but effective electrochemical signal amplification strategy for intracellular ATP detection, which replaces the regular ATP aptamer-linked ferrocene monomer with a polymer, thus greatly magnifying the amounts of electrochemical reporters linked to one chain of the aptamer and enhancing the signals. This ferrocene polymer–ATP aptamer was further immobilized onto Au nanowire electrodes (SiC@C@Au NWEs) to achieve accurate quantification of intracellular ATP in single cells, presenting high electrochemical signal output and high specificity. This work not only provides a powerful tool for quantifying intracellular ATP but also offers a simple and versatile strategy for electrochemical signal amplification in the detection of broader non-electroactive molecules involved in different kinds of intracellular physiological processes.

Graphical abstract: Electroactive polymer tag modified nanosensors for enhanced intracellular ATP detection

Supplementary files

Article information

Article type
Paper
Submitted
04 apr 2024
Accepted
29 apr 2024
First published
01 mai 2024

Analyst, 2024,149, 3530-3536

Electroactive polymer tag modified nanosensors for enhanced intracellular ATP detection

Y. Kang, Y. Jiao, C. Zhao, X. Zhang and W. Huang, Analyst, 2024, 149, 3530 DOI: 10.1039/D4AN00511B

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