Issue 9, 2023

Heterogeneous tissue construction by on-demand bubble-assisted acoustic patterning

Abstract

Highly heterogeneous structures are closely related to the realization of the tissue functions of living organisms. However, precisely controlling the assembly of heterogeneous structures is still a crucial challenge. This work presents an on-demand bubble-assisted acoustic method for active cell patterning to achieve high-precision heterogeneous structures. Active cell patterning is achieved by the combined effect of acoustic radiation forces and microstreaming around oscillating bubble arrays. On-demand bubble arrays allow flexible construction of cell patterns with a precision of up to 45 μm. As a typical example, the in vitro model of hepatic lobules, composed of patterned endothelial cells and hepatic parenchymal cells, was constructed and cultured for 5 days. The good performance of urea and albumin secretion, enzymatic activity and good proliferation of both cells prove the feasibility of this technique. Overall, this bubble-assisted acoustic approach provides a simple and efficient strategy for on-demand large-area tissue construction, with considerable potential for different tissue model fabrication.

Graphical abstract: Heterogeneous tissue construction by on-demand bubble-assisted acoustic patterning

Supplementary files

Article information

Article type
Paper
Submitted
12 feb 2023
Accepted
26 mar 2023
First published
27 mar 2023

Lab Chip, 2023,23, 2206-2216

Heterogeneous tissue construction by on-demand bubble-assisted acoustic patterning

Q. Hu, X. Hu, Y. Shi, L. Liang, J. Zhu, S. Zhao, Y. Wang, Z. Wu, F. Wang, F. Zhou and Y. Yang, Lab Chip, 2023, 23, 2206 DOI: 10.1039/D3LC00122A

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